Modifying Enzymes

Proteins that have the ability to structurally alter macromolecules without being altered themselves. Useful for a variety of molecular biology procedures; products include DNA and RNA polymerases, phosphatases, kinases, nucleases, etc.

Search Within Results

316 – 330 of 2,660 results

316

Promega Pepsin

Pepsin preferentially cleaves at the C-terminus of phenylalanine, leucine, tyrosine and tryptophan. Pepsin can be used alone or with other proteases for protein analysis by mass spectrometry and other applications. Activity is optimal at pH 1.0-3.0.

Pricing & Availability

317

Promega Elastase

Elastase is a serine protease that preferentially cleaves at the C-terminus of alanine, valine, serine, glycine, leucine or isoleucine. Elastase has a unique capability of digesting elastin. Elastase activity is optimal at pH 9.0.

Pricing & Availability

318

Promega SP6 RNA Polymerase

DNA-dependent RNA polymerase for in vitro transcription.

Pricing & Availability

319

Promega T4 Polynucleotide Kinase

T4 PNK catalyzes the transfer of the gamma-phosphate from ATP to the 5'-terminus of polynucleotides or to mononucleotides bearing a 5'-hydroxyl group.

Pricing & Availability
Specifications

Components	10X Reaction Buffer: 700mM Tris HCl (pH 7.6 at 25°C), 100mM MgCl2, 50mM DTT
Content And Storage	-30°C to -10°C
Inhibitors	Ammonium Ions and low levels of Phosphate Buffers
Form	Liquid
Product Type	T4 Polynucleotide Kinase
Storage Buffer	20mM Tris HCl (pH 7.5), 25mM KCl, 2mM DTT, 0.1mM EDTA, 0.1μM ATP, 50% Glycerol
Molecular Weight (g/mol)	132
Color	Colorless
pH	7.5
Purity	≥90%
Concentration	5 to 10 U/μL
For Use With (Application)	5′ End-labeling of single or double-stranded DNA and RNA molecules for use as probes, sequencing or DNA Protein footprinting, Phosphorylation of DNA prior to cloning
Source	Recombinant E. coli strain

320

Promega 4-CORE™ Buffer Pack

Consists of one vial each of Restriction Enzyme Buffers A, B, C and D.

Pricing & Availability

321

Promega T4 DNA Ligase Buffer Pack

For ligation of DNA ends.

Pricing & Availability

322

Promega Ribonuclease H

RNase H is an endonuclease that specifically hydrolyzes the phosphodiester bonds of RNA hybridized to DNA.

Pricing & Availability
Specifications

Content And Storage	-30°C to -10°C
Form	Liquid
Product Type	Ribonuclease H
Storage Buffer	20mM HEPES-KOH (pH 7.8 at 25°C), 1mM DTT, 50mM KCl, 0.2mg/mL BSA, 50% Glycerol
Color	Colorless
pH	7.8
Concentration	0.5 to 2 U/μL
For Use With (Application)	Removal of the RNA strand prior to second strand cDNA synthesis, analysis of in-vitro polyadenylation reaction products, microarrays
Source	Recombinant E. coli strain

323

Promega Modifying Enzymes: Single-Stranded DNA Binding Protein

Binds with high affinity in a cooperative manner to single-stranded DNA but does not bind well to double-stranded DNA

Pricing & Availability

324

Promega MULTI-CORE™ Buffer Pack

Universal restriction enzyme buffer. Supports double-digests with many restriction enzymes.

Pricing & Availability

325

Promega S1 Nuclease

Endonuclease specific for single-stranded RNA or DNA.

Pricing & Availability

326

Promega T7 RNA Polymerase

DNA-dependent RNA polymerase for in vitro transcription.

Pricing & Availability
Specifications

Components	5X Reaction Buffer: 100mM DTT and transcription optimized 5X Buffer: 200mM Tris HCl (pH 7.9 at 25°C), 30mM MgCl2, 10mM Spermidine, 50mM NaCl
Content And Storage	-30°C to -10°C
Inhibitors	Recombinant RNasin™ Ribonuclease
Form	Liquid
Product Type	T7 RNA Polymerase
Storage Buffer	20mM Potassium Phosphate (pH 7.7 at 25°C), 1mM EDTA, 10mM DTT, 100mM NaCl, 0.1% Triton™ X-100, 50% Glycerol
Color	Colorless
pH	7.7
Purity	>90%
For Use With (Application)	Generation of templates for in-vitro translation, generation of probes for Nucleic Acid hybridization, generation of RNA processing Substrates, generation of Antisense RNA
Source	Recombinant E. coli strain

327

Promega Streptavidin Alkaline Phosphatase

Used for the detection of biotinylated molecules.

Pricing & Availability

328

Promega T3 RNA Polymerase

DNA-dependent RNA polymerase for in vitro transcription.

Pricing & Availability
Specifications

Components	100mM DTT 5X Reaction Buffer, transcription optimized 5X Buffer: 200mM Tris HCl (pH 7.9 at 25°C), 30mM MgCl2, 10mM Spermidine, 50mM NaCl
Content And Storage	-30°C to -10°C
Form	Liquid
Product Type	T3 RNA Polymerase
Storage Buffer	20mM Potassium Phosphate (pH 7.7 at 25°C), 1mM EDTA, 10mM DTT, 100mM NaCl, 0.1% Triton™ X-100, 50% Glycerol
Color	Colorless
pH	7.7
Purity	>90%
For Use With (Application)	Generation of templates for in-vitro translation, generation of probes for Nucleic Acid hybridization, generation of RNA processing Substrates, generation of Antisense RNA
Source	Recombinant E. coli strain

329

Promega T4 DNA Ligase

For ligation of DNA ends.

Pricing & Availability
Specifications

Components	10X Reaction Buffer: 300mM Tris HCl (pH 7.8 at 25°C), 100mM MgCl2, 100mM DTT and 10mM ATP
Content And Storage	-30°C to -10°C
Form	Liquid
Product Type	T4 DNA Ligase
Storage Buffer	10mM Tris HCl (pH 7.4 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 50% Glycerol
Molecular Weight (g/mol)	68
Color	Colorless
pH	7.4
Purity	>90%
For Use With (Application)	Joining double-stranded DNA molecules with cohesive or blunt ends (5', 3' or blunt-ended DNA inserts)
Source	Recombinant E. coli strain

330

Promega T4 RNA Ligase

T4 RNA Ligase catalyzes the ATP-dependent ligation of single-stranded RNA or DNA onto the 5'-phosphoryl termini of single-stranded RNA or DNA.

Pricing & Availability

Modifying Enzymes

Filtered Search Results

Narrow Results